Two recent isolates of Akabane virus from Oman were subjected to detailed biochemical analysis. The RNA and protein profiles were analysed by polyacrylamide gel electrophoresis and formaldehyde-agarose gel electrophoresis and compared with other prototype reference strains. In contrast to previous reports, the biochemical analysis indicated that the viral mRNAs were polyadenylated. The virus specific proteins were indistinguishable between different Akabane strains, but differences were observed in their genomic profiles which could be used to distinguish them from previously isolated prototype reference strains. Genome analysis may be a useful means of identifying different Akabane isolates for epidemiological studies.
Two recent isolates of Akabane virus from Oman were subjected to detailed biochemical analysis. The RNA and protein profiles were analysed by polyacrylamide gel electrophoresis and formaldehyde-agarose gel electrophoresis and compared with other prototype reference strains. In contrast to previous r...
مادة فرعية
Journal of Applied Animal Research