Pteropyrum scoparium (P. scoparium, Sidaf) is a medicinally important plant available in most of the tropical countries including Sultanate of Oman and locally it is used to treat dyspepsia and blood purifying agent. The aim of the present study is to prepare leave extracts of different polarities and to evaluate their phytochemicals, total phenols content, antioxidant and antimicrobial activities. The leaves of the plant were extracted with absolute ethanol by maceration method at ambient temperature for one week. The extract was filtered and evaporated to dryness by using rotary evaporator. The residue was suspended in equal volume of ethanol: water and fractionation with hexane, chloroform, and butanol, respectively. The total phenols content, antioxidant and antimicrobial activities of four prepared extracts were evaluated by Folin-Ciocalteu reagent (FCR), 2,2-diphenyl1-picrylhydrazyl (DPPH) and agar disc diffusion methods. The highest amount of phenols content was found in hydro alcoholic extract and the lowest content was in hexane to be in the order of hydro alcohol > butanol > chloroform > hexane extract. The main antioxidant activity was found in residue from butanol and the minimum activity was in that from chloroform, the order of antioxidant activity; butanol > hydro alcoholic > hexane > chloroform extract. The antibacterial activity of ethanol extract and its derived fractions of the plant against one Gram-positive Staphylococcus aureus (S. aureus) and one Gram-negative Escherichia coli (E. coli) bacteria strains were qualitatively assessed by the presence or absence of inhibition zones. All the extracts of the selected plant at most of the concentrations did not exhibit potent antibacterial activity. The phytochemical studies of the prepared extracts, showed that all the extract contains saponins, steroids, and terpenoids. In addition to that, the chloroform, butanol and hydro-alcohol extract contains tannins and glycoside. In conclusion, all the crude extracts showed significant antioxidant activity. It could be used as natural antioxidant instead of synthetic one

Pteropyrum scoparium (P. scoparium, Sidaf) is a medicinally important plant available in most of the tropical countries including Sultanate of Oman and locally it is used to treat dyspepsia and blood purifying agent. The aim of the present study is to prepare leave extracts of different polarities...