ورقة بحثية
استهلاك الأوكسجين فى المايتوكوندريا من قبل القلفة و الخلايا الليفية = Mitochondrial Oxygen Consumption by the Foreskin and its Fibroblast-rich Culture

Al Jasmi, Fatma.


 

استهلاك الأوكسجين فى المايتوكوندريا من قبل القلفة و الخلايا الليفية = Mitochondrial Oxygen Consumption by the Foreskin and its Fibroblast-rich Culture

Al Jasmi, Fatma.

Objectives: This study investigated the feasibility of using a phosphorescence oxygen analyser to measure cellular respiration (mitochondrial O2 consumption) in foreskin samples and their fibroblast-rich cultures. Methods: Foreskin specimens from normal infants were collected immediately after circumcision and processed for measuring cellular respiration and for culture. Cellular mitochondrial O2 consumption was determined as a function of time from the phosphorescence decay of the Pd (II) meso-tetra-(4-sulfonatophenyl)-tetrabenzoporphyrin. Results: In sealed vials containing a foreskin specimen and glucose, O2 concentration decreased linearly with time, confirming the zero-order kinetics of O2 consumption by cytochrome oxidase. Cyanide inhibited O2 consumption, confirming that the oxidation occurred mainly in the mitochondrial respiratory chain. The rate of foreskin respiration (mean ± SD) was 0.074 ± 0.02 μM O2 min-1 mg-1 (n = 23). The corresponding rate for fibroblast-rich cultures was 9.84 ± 2.43 μM O2 min-1 per 107 cells (n = 15). Fibroblast respiration was significantly lower in a male infant with dihydrolipoamide dehydrogenase gene mutations, but normalised with the addition of thiamine or carnitine. Conclusion: The foreskin and its fibroblast-rich culture are suitable for assessment of cellular respiration. However, the clinical utility of foreskin specimens to detect disorders of impaired cellular bioenergetics requires further investigation.

Objectives: This study investigated the feasibility of using a phosphorescence oxygen analyser to measure cellular respiration (mitochondrial O2 consumption) in foreskin samples and their fibroblast-rich cultures. Methods: Foreskin specimens from normal infants were collected immediately after cir...

مادة فرعية

المؤلف : Al Jasmi, Fatma.

مؤلف مشارك : Thachillath Pramathan
Adnan Swid
Bahjat Sahari
Harvey S. Penefsky
Abdul-Kader Souid

بيانات النشر : Muscat، Sultanate of Oman : Sultan Qaboos University / College of Medicine and Health Sciences، 2013مـ.

التصنيف الموضوعي : العلوم التطبيقية|العلوم الطبية .

المواضيع : Cellular respiration .

التنفس الخلوي .

Oxygen .

الأوكسجين .

رقم الطبعة : 3

المصدر : Sultan Qaboos University / College of Medicine and Health Sciences : Muscat، Sulatante of Oman.

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