ورقة بحثية
تحديد الطفرات المسببة لمرض بومبي في عينات أنسجة محفوظة وتطوير الفحص الجزيئي= The Identification of Pompe Disease Mutations in Archival Tissues and Development of a Rapid Molecular-based Test

Al Ansar, Aliya S.


 

تحديد الطفرات المسببة لمرض بومبي في عينات أنسجة محفوظة وتطوير الفحص الجزيئي= The Identification of Pompe Disease Mutations in Archival Tissues and Development of a Rapid Molecular-based Test

Al Ansar, Aliya S.

Objectives: Pompe disease (glycogen storage disease type II) is a rare autosomal recessive lysosomal storage disease that is caused by acid alpha-glucosidase deficiency. Early enzyme replacement therapy can benefit infants with the disease but the diagnosis is complicated by the rarity of the disease and the heterogeneity of the clinical manifestations. In this study, DNA extracted from archival postmortem formalin-fixed paraffin-embedded tissues was used to identify Pompe disease mutations in Oman and develop a rapid molecular-based test. Methods: Intronic primers were designed to amplify short fragments (193–454 base pairs [bp]) from coding exons (2–20) and screen for mutations using direct sequencing (DS). Results: Two mutations known to cause severe disease were identified in two samples. One was a coding mutation, c.2560C>T (p.Arg854X), and the second was found at a splice acceptor site, c.1327-2A>G. Polymerase chain reaction- and restriction fragment length polymorphism-based tests were designed for the rapid genotyping of the identified mutations. Conclusion: These tests can facilitate prenatal diagnosis and help in identifying carriers in families with the identified mutations.

Objectives: Pompe disease (glycogen storage disease type II) is a rare autosomal recessive lysosomal storage disease that is caused by acid alpha-glucosidase deficiency. Early enzyme replacement therapy can benefit infants with the disease but the diagnosis is complicated by the rarity of the dise...

مادة فرعية

المؤلف : Al Ansar, Aliya S.

مؤلف مشارك : Al Rawahi, Samira
Taher Ba-Omar
Al Nabhani, Mariam
Anand Date

بيانات النشر : Muscat، Sultanate of Oman : Sultan Qaboos University / College of Medicine and Health Sciences، 2013مـ.

التصنيف الموضوعي : العلوم التطبيقية|العلوم الطبية .

المواضيع : Pompe disease - Sultanate of Oman.

Genetics .

الوراثة، علم .

رقم الطبعة : 4

المصدر : Sultan Qaboos University / College of Medicine and Health Sciences : Muscat، Sulatante of Oman.

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