ورقة بحثية
An Electrophoretic Study of LDH-Isozyme Distribution in Developing Embryonic and Adult Tissues of the Toad Bufo arabicus = دراسة استشرادية لتوزيع ايسوزوم LDH في مراحل نمو جنين وأعضاء الضفدع العربي البالغ

Ambusaidi, Intisar.


 

An Electrophoretic Study of LDH-Isozyme Distribution in Developing Embryonic and Adult Tissues of the Toad Bufo arabicus = دراسة استشرادية لتوزيع ايسوزوم LDH في مراحل نمو جنين وأعضاء الضفدع العربي البالغ

Ambusaidi, Intisar.

Lactate (LDH) dehydrogenases were studied by electrophoresis to investigate the possibility of isozymic differences in different stages and organs of B. arabicus embryos, tadpoles and adults. The expression pattern of lactate dehydrogenase (LDH) isozymes was analysed using nondenaturing discontinuous polyacrylamide gel electrophoresis (PAGE). Five LDH isozymes were observed in five different adult tissues. The most cathodal isozyme LDH-5 (A4) showed the strongest activity. All embryonic stages tested displayed the anodic isozymes first. Four LDH isozymes were present in the stages 1-16 (fertilized egg-tail bud stage). The first appearance of the fifth isozyme was detected in stage 17 when the muscular response started. This isozyme showed gradual increase in intensity from stage 17 to stage 43.

Lactate (LDH) dehydrogenases were studied by electrophoresis to investigate the possibility of isozymic differences in different stages and organs of B. arabicus embryos, tadpoles and adults. The expression pattern of lactate dehydrogenase (LDH) isozymes was analysed using nondenaturing discontinuo...

مادة فرعية

المؤلف : Ambusaidi, Intisar.

مؤلف مشارك : Taher Ba-Omar
Al Bahri. Saif
AlShihi, Aisha

بيانات النشر : Muscat، Sultanate of Oman : Sultan Qaboos Journal of Science، 2009مـ.

التصنيف الموضوعي : العلوم البحتة| .

المواضيع : Biology .

Frogs .

الأحياء .

الضفادع .

رقم الطبعة : 1

المصدر : Sultan Qaboos University : Muscat، Sultanate of Oman.

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